Bulked Segregant Analysis to Detect Main Effect of QTL Associated with Sheath Blight Resistance in BPT-5204/ARC10531 Rice (Oryza sativa L)

The population consisting of 210 F2:3 individuals from the cross between BPT-5204 (highly susceptible to sheath blight) and ARC-10531 a land race from Assam (moderately resistant to sheath blight) was analyzed to identify the markers associated with sheath blight resistance and to study any association of any morphological trait to disease incidence. The frequency distribution curve of F2:3 progenies for disease trait were continuous, indicating the polygenic control over the trait. The range of relative lesion height was 21-75% with a mean of 38.59%.No significant association between sheath blight disease and other morphological traits were detected in F2:3 populations. Parental polymorphisms were surveyed with 500 primer pairs of simple sequence repeats (SSR), revealed 70 polymorphic markers between the parents. In order to detect the major effect, QTL associated with sheath blight resistance, a strategy of combining the DNA pooling from selected segregants and genotyping was adopted. The association of putative markers identified based on DNA pooling from selected segregant was established by Single Marker Analysis (SMA).The results of SMA revealed that SSR markers, RM336 (chromosome#7) and RM205 (chromosome#9) showed significant association with sheath blight and accounted for 21.8% and 17.3% of total variation respectively. The results obtained from the DNA pooling of phenotypic extremes could be a useful strategy to detect the genetic loci with major effects of the complex trait such as disease resistance in rice. *Corresponding author: Shailesh Yadav, Institute of Biotechnology, Acharya N G Ranga Agricultural University, Rajendranagar, Hyderabad, India, Tel: +917893473471; E-mail: [email protected] Received August 22, 2015; Accepted September 28, 2015; Published October 01, 2015 Citation: Yadav S, Anuradha G, Kumar RR, Vemireddy LR, Sudhakar R, et al. (2015) Bulked Segregant Analysis to Detect Main Effect of QTL Associated with Sheath Blight Resistance in BPT-5204/ARC10531 Rice (Oryza sativa L). J Rice Res 3: 149. doi:10.4172/2375-4338.1000149 Copyright: © 2015 Yadav S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.